OpenBiome

GMbC Starter Kit

Name: GMbC Starter Kit
Brand: OpenBiome
Price: 1070.0 USD
Availability: InStock

GMbC Starter kits are prepared by the Global Microbiome Conservancy (GMbC) program at OpenBiome as part of our nonprofit mission to advance understanding of the human microbiome and promote inclusive science. Each kit contains 48 anaerobic bacterial seeds stocks derived from stool samples donated by diverse, highly underrepresented communities around the world that reflect both rural and urban lifestyles and diets.

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Format and Storage

GMbC Starter Kit contains frozen bacterial isolate suspensions in 20 ul of freezer broth (reduced brain heart infusion with 0.1% L-cysteine, 20% glycerol).
Material will be delivered on dry ice via overnight courier with temperature verification. Upon receipt, GMbC Starter Kit should be stored -80°C or colder.

Handling Instructions

To culture bacterial isolates, we recommend an anaerobic atmosphere (5% hydrogen, 5% carbon dioxide, 90% nitrogen), reduced Brucella agar (5% laked sheep’s blood, supplemented with hemin and vitamin K) and incubation at 35° to 37° °C for all isolates.
When handling GMbC Starter Kit packaging and the aliquots contained within, always wear appropriate personal protective equipment.
Contact email: info@openbiome.org/phone:(617) 575-2201 immediately if package or product integrity has been compromised.

Citations

Groussin M, Poyet M, Sistiaga A, Kearney SM, Moniz K, Noel M, Hooker J, Gibbons SM, Segurel L, Froment A, Mohamed RS, Fezeu A, Juimo VA, Lafosse S, Tabe FE, Girard C, Iqaluk D, Nguyen LTT, Shapiro BJ, Lehtimäki J, Ruokolainen L, Kettunen PP, Vatanen T, Sigwazi S, Mabulla A, Domínguez-Rodrigo M, Nartey YA, Agyei-Nkansah A, Duah A, Awuku YA, Valles KA, Asibey SO, Afihene MY, Roberts LR, Plymoth A, Onyekwere CA, Summons RE, Xavier RJ, Alm EJ. Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell. 2021 Apr 15;184(8):2053-2067.e18. doi: 10.1016/j.cell.2021.02.052. Epub 2021 Mar 31. PMID: 33794144.

Safety Information

Researchers should only work with GMbC Starter Kit materials in a certified biosafety cabinet within a BSL-2 rated facility. Upon delivery, always examine packaging integrity and shipping temperature indicator.

Legal Information

Bacterial isolates are intended for laboratory research use only. This product is not for use in humans. For further restrictions on use and applicable law, please see the Material Transfer Agreement (MTA).

GMbC Program Biobanking

GMbC program biobanking is performed in close collaboration with an international consortium of researchers and participating communities in accordance with our mission to advance understanding of the microbiome and inclusive science.

We are committed to establishing equitable collaborations and work to grow research capacity, as needed, of our scientific collaborators in low-and middle income countries, and implement benefit sharing programs for communities providing microbiome samples. Our biobanking is overseen by the GMbC program’s Science and Ethics Advisory Board, and complies with international regulations including the Convention on Biological Diversity and Nagoya Protocol.

For more information, please read our list of guiding principles.

Bacterial Isolates

You can find an excel sheet for all 48 bacterial isolates here

Genus Species	Isolate ID	Participant Lifestyle	Publication Status
Enterococcus_hirae	3790QQ_0218_002_B5	Rural	N/A
Bifidobacterium_dentium	3790QQ_0218_002_C8	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Bacteroides_fragilis	3790QQ_0218_002_G10	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Lactobacillus_mucosae	3790QQ_0218_002_G2	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Coprococcus_comes	3401SE_0218_027_F9	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Bacteroides_intestinalis	8131GG_1118_056_A5	Urban	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Bacteroides_vulgatus	3401SE_0218_027_B2	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Bacteroides_uniformis	3401SE_0218_027_D3	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Blautia_wexlerae	3050YG_0918_062_C1	Intermediate	N/A
Prevotella_copri	3401SE_0218_027_D8	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Bacteroides_ovatus	3401SE_0218_027_F1	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Odoribacter_splanchnicus	3401SE_0218_027_G10	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Fusobacterium_A_varium	3790QQ_0218_017_F8	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Ruminococcus_faecis	3401SE_0218_027_H11	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Butyricimonas_virosa	3401SE_0218_027_H4	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Clostridium_perfringens	8144JZ_0517_029_D11	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Clostridium_baratii	4324HL_0218_028_C11	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Enterobacter_cloacae	4324HL_0218_028_F10	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Lactobacillus_ruminis	8144JZ_0517_029_A3	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Enterococcus_saccharolyticus	8144JZ_0517_029_A7	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Bifidobacterium_adolescentis	8144JZ_0517_029_B4	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Dorea_formicigenerans	1462YY_0218_030_H9	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Paraclostridium_bifermentans	8144JZ_0517_029_F10	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Dielma_fastidiosa	1462QI_0319_071_D07	Rural	N/A
Streptococcus_infantarius	1462YY_0218_030_C1	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Eubacterium_rectale	3050YG_0918_062_H7	Intermediate	N/A
Bacteroides_caccae	1462YY_0218_030_C7	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Parabacteroides_merdae	1462YY_0218_030_D5	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Parabacteroides_distasonis	1462YY_0218_030_F1	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Coprococcus_catus	1462YY_0218_030_G6	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Barnesiella_intestinihominis	8131GG_1118_056_B12	Urban	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Alistipes_putredinis	8131GG_1118_056_B8	Urban	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Bacteroides_xylanisolvens	8131GG_1118_056_C4	Urban	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Bacteroides_faecichinchillae	8131GG_1118_056_D9	Urban	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Holdemanella_biformis	9734KT_0319_070_F07	Rural	N/A
Alistipes_shahii	8131GG_1118_056_F8	Urban	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Phascolarctobacterium_faecium	4452YQ_0918_057_A12	Urban	N/A
Roseburia_inulinivorans	3606MB_1118_055_G3	Intermediate	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Streptococcus_gallolyticus	4452YQ_0918_057_F4	Urban	N/A
Collinsella_aerofaciens	4452YQ_0918_057_G6	Urban	N/A
Enterococcus_faecium	3050YG_0918_062_A3	Intermediate	N/A
Streptococcus_lutetiensis	3050YG_0918_062_C2	Intermediate	N/A
Bilophila_wadsworthia	9734KT_0319_070_F04	Rural	N/A
Escherichia_coli	3790QQ_0218_002_A4	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).
Ruthenibacterium_lactatiformans	1462QI_0319_071_D05	Rural	N/A
Bacteroides_nordii	1462QI_0319_071_B05	Rural	N/A
Clostridium_symbiosum	1462QI_0319_071_C06	Rural	N/A
Cetobacterium_A_somerae	3790QQ_0218_017_C2	Rural	Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

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