OpenBiome

GMbC Starter Kit

GMbC Starter Kit

GMbC Starter kits are prepared by the Global Microbiome Conservancy (GMbC) program at OpenBiome as part of our nonprofit mission to advance understanding of the human microbiome and promote inclusive science. Each kit contains 48 anaerobic bacterial seeds stocks derived from stool samples donated by diverse, highly underrepresented communities around the world that reflect both rural and urban lifestyles and diets. 

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Format and Storage

  • GMbC Starter Kit contains frozen bacterial isolate suspensions in 20 ul of freezer broth (reduced brain heart infusion with 0.1% L-cysteine, 20% glycerol).
  • Material will be delivered on dry ice via overnight courier with temperature verification. Upon receipt, GMbC Starter Kit should be stored -80°C or colder.

Handling Instructions

  • To culture bacterial isolates, we recommend an anaerobic atmosphere (5% hydrogen, 5% carbon dioxide, 90% nitrogen), reduced Brucella agar (5% laked sheep’s blood, supplemented with hemin and vitamin K) and incubation at 35° to 37° °C for all isolates.
  • When handling GMbC Starter Kit packaging and the aliquots contained within, always wear appropriate personal protective equipment.
  • Contact email: info@openbiome.org/phone:(617) 575-2201 immediately if package or product integrity has been compromised.

Safety Information

Researchers should only work with GMbC Starter Kit materials in a certified biosafety cabinet within a BSL-2 rated facility. Upon delivery, always examine packaging integrity and shipping temperature indicator.

Legal Information

Bacterial isolates are intended for laboratory research use only. This product is not for use in humans. For further restrictions on use and applicable law, please see the Material Transfer Agreement (MTA).

GMbC Program Biobanking

GMbC program biobanking is performed in close collaboration with an international consortium of researchers and participating communities in accordance with our mission to advance understanding of the microbiome and inclusive science.

We are committed to establishing equitable collaborations and work to grow research capacity, as needed, of our scientific collaborators in low-and middle income countries, and implement benefit sharing programs for communities providing microbiome samples. Our biobanking is overseen by the GMbC program’s Science and Ethics Advisory Board, and complies with international regulations including the Convention on Biological Diversity and Nagoya Protocol.

For more information, please read our list of guiding principles.

Bacterial Isolates

You can find an excel sheet for all 48 bacterial isolates here

Genus Species Isolate ID Participant Location Participant Lifestyle Publication Status
Enterococcus_hirae
3790QQ_0218_002_B5 Cameroon Rural

N/A

Bifidobacterium_dentium
3790QQ_0218_002_C8 Cameroon Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Bacteroides_fragilis
3790QQ_0218_002_G10 Cameroon Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Lactobacillus_mucosae
3790QQ_0218_002_G2 Cameroon Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Coprococcus_comes
3401SE_0218_027_F9 Tanzania Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Bacteroides_intestinalis
8131GG_1118_056_A5 Nigeria Urban

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Bacteroides_vulgatus
3401SE_0218_027_B2 Tanzania Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Bacteroides_uniformis
3401SE_0218_027_D3 Tanzania Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Blautia_wexlerae
3050YG_0918_062_C1 Rwanda Intermediate

N/A

Prevotella_copri
3401SE_0218_027_D8 Tanzania Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Bacteroides_ovatus
3401SE_0218_027_F1 Tanzania Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Odoribacter_splanchnicus
3401SE_0218_027_G10 Tanzania Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Fusobacterium_A_varium
3790QQ_0218_017_F8 Cameroon Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Ruminococcus_faecis
3401SE_0218_027_H11 Tanzania Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Butyricimonas_virosa
3401SE_0218_027_H4 Tanzania Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Clostridium_perfringens
8144JZ_0517_029_D11 Cameroon Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Clostridium_baratii
4324HL_0218_028_C11 Cameroon Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Enterobacter_cloacae
4324HL_0218_028_F10 Cameroon Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Lactobacillus_ruminis
8144JZ_0517_029_A3 Cameroon Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Enterococcus_saccharolyticus
8144JZ_0517_029_A7 Cameroon Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Bifidobacterium_adolescentis
8144JZ_0517_029_B4 Cameroon Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Dorea_formicigenerans
1462YY_0218_030_H9 Tanzania Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Paraclostridium_bifermentans
8144JZ_0517_029_F10 Cameroon Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Dielma_fastidiosa
1462QI_0319_071_D07 Malaysia Rural

N/A

Streptococcus_infantarius
1462YY_0218_030_C1 Tanzania Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Eubacterium_rectale
3050YG_0918_062_H7 Rwanda Intermediate

N/A

Bacteroides_caccae
1462YY_0218_030_C7 Tanzania Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Parabacteroides_merdae
1462YY_0218_030_D5 Tanzania Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Parabacteroides_distasonis
1462YY_0218_030_F1 Tanzania Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Coprococcus_catus
1462YY_0218_030_G6 Tanzania Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Barnesiella_intestinihominis
8131GG_1118_056_B12 Nigeria Urban

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Alistipes_putredinis
8131GG_1118_056_B8 Nigeria Urban

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Bacteroides_xylanisolvens
8131GG_1118_056_C4 Nigeria Urban

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Bacteroides_faecichinchillae
8131GG_1118_056_D9 Nigeria Urban

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Holdemanella_biformis
9734KT_0319_070_F07 Malaysia Rural

N/A

Alistipes_shahii
8131GG_1118_056_F8 Nigeria Urban

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Phascolarctobacterium_faecium
4452YQ_0918_057_A12 Ghana Urban

N/A

Roseburia_inulinivorans
3606MB_1118_055_G3 Nigeria Intermediate

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Streptococcus_gallolyticus
4452YQ_0918_057_F4 Ghana Urban

N/A

Collinsella_aerofaciens
4452YQ_0918_057_G6 Ghana Urban

N/A

Enterococcus_faecium
3050YG_0918_062_A3 Rwanda Intermediate

N/A

Streptococcus_lutetiensis
3050YG_0918_062_C2 Rwanda Intermediate

N/A

Bilophila_wadsworthia
9734KT_0319_070_F04 Malaysia Rural

N/A

Escherichia_coli
3790QQ_0218_002_A4 Cameroon Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

Ruthenibacterium_lactatiformans
1462QI_0319_071_D05 Malaysia Rural

N/A

Bacteroides_nordii
1462QI_0319_071_B05 Malaysia Rural

N/A

Clostridium_symbiosum
1462QI_0319_071_C06 Malaysia Rural

N/A

Cetobacterium_A_somerae
3790QQ_0218_017_C2 Cameroon Rural

Elevated rates of horizontal gene transfer in the industrialized human microbiome. Cell 184, 2053-2067 (2021).

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